Details of experiments between Q6P0Q8 and Q82005
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: MAST2
Protein A fragment: 1102-1193
Protein A site: PDZ
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: HPV73-E6
Protein B fragment: 139-148
Protein B site: PBM
Protein B sequence: SGSRPSATVV
Protein B construct: biotin-ttds-SGSRPSATVV
Measured dissociation constant (10-6M): 103.87
Standard deviation of dissociation constant (10-6M): 11.58
Experiment method: competitive FP
Details of affinity fitting: Competitive binding equation in ProFit
PUBMED ID of publication: 36115835
Number of measurements: 3
Measured pKd: 3.98
Experiment series 2
Protein A protein: MAST2
Protein A fragment: 1102-1193
Protein A site: PDZ
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: HPV73-E6
Protein B fragment: 139-148
Protein B site: PBM
Protein B sequence: SGSRPSATVV
Protein B construct: biotin-ttds-SGSRPSATVV
Average holdup BI: 0.1
Immobilized partner concentration in holdup experiment (10-6M): 10
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 4.05
Experiment series 3
Protein A protein: MAST2
Protein A fragment: 1102-1193
Protein A site: PDZ
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: HPV73-E6
Protein B fragment: 139-148
Protein B site: PBM
Protein B sequence: SGSRPSATVV
Protein B construct: biotin-ttds-SGSRPSATVV
Average holdup BI: 0.12
Normalized holdup BI: 0.12
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 3.88