Details of experiments between Q6P0Q8 and P59637
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: MAST2
Protein A fragment: 1102-1193
Protein A site: PDZ
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: SARS-Cov-E
Protein B fragment: 67-76
Protein B site: PBM
Protein B sequence: SSEGVPDLLV
Protein B construct: Biotin-(PEG)3-NLNSSEGVPDLLV
Average holdup BI: 0.08
Holdup BI standard deviation: 0.03
Immobilized partner concentration in holdup experiment (10-6M): 20
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 2
The affinity was below the detection threshold of the assay.
Experiment series 2
Protein A protein: MAST2
Protein A fragment: 1102-1193
Protein A site: PDZ
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: SARS-Cov-E
Protein B fragment: 67-76
Protein B site: PBM
Protein B sequence: SSEGVPDLLV
Protein B construct: Biotin-(PEG)3-NLNSSEGVPDLLV
Measured dissociation constant (10-6M): 252.7
Standard deviation of dissociation constant (10-6M): 22.76
Experiment method: competitive FP
Details of affinity fitting: Competitive binding equation in ProFit
PUBMED ID of publication: 36115835
Number of measurements: 3
Measured pKd: 3.6