Details of experiments between Q6P0Q8 and O75084
Note that the results of all experiments are listed, regardless of the modification states of the fragments.



Experiment series 1

Protein A protein: MAST2
Protein A fragment: 1102-1193
Protein A site: PDZ
Protein A construct: his6-MBP-TEVsite-PDZ

Protein B protein: FZD7
Protein B fragment: 565-574
Protein B site: PBM
Protein B sequence: SHSSKGETAV
Protein B construct: biotin-ttds-SHSSKGETAV

Measured dissociation constant (10-6M): 10.79
Standard deviation of dissociation constant (10-6M): 0.9
Experiment method: competitive FP
Details of affinity fitting: Competitive binding equation in ProFit

PUBMED ID of publication: 36115835
Number of measurements: 3
Measured pKd: 4.97


Experiment series 2

Protein A protein: MAST2
Protein A fragment: 1102-1193
Protein A site: PDZ
Protein A construct: his6-MBP-TEVsite-PDZ

Protein B protein: FZD7
Protein B fragment: 565-574
Protein B site: PBM
Protein B sequence: SHSSKGETAV
Protein B construct: biotin-ttds-SHSSKGETAV

Average holdup BI: 0.27
Immobilized partner concentration in holdup experiment (10-6M): 6
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 4.88


Experiment series 3

Protein A protein: MAST2
Protein A fragment: 1102-1193
Protein A site: PDZ
Protein A construct: his6-MBP-TEVsite-PDZ

Protein B protein: FZD7
Protein B fragment: 565-574
Protein B site: PBM
Protein B sequence: SHSSKGETAV
Protein B construct: biotin-ttds-SHSSKGETAV

Average holdup BI: 0.7
Normalized holdup BI: 0.7
Immobilized partner concentration in holdup experiment (10-6M): 21.5
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 5.09