Details of experiments between Q68DX3 and Q9IR59
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: FRMPD2
Protein A fragment: 770-861
Protein A site: PDZ_1
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: HPV82-E6
Protein B fragment: 142-151
Protein B site: PBM
Protein B sequence: PPRQRSETQV
Protein B construct: biotin-ttds-PPRQRSETQV
Average holdup BI: -0.05
Immobilized partner concentration in holdup experiment (10-6M): 10
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 2
Protein A protein: FRMPD2
Protein A fragment: 944-1038
Protein A site: PDZ_2
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: HPV82-E6
Protein B fragment: 142-151
Protein B site: PBM
Protein B sequence: PPRQRSETQV
Protein B construct: biotin-ttds-PPRQRSETQV
Average holdup BI: -0.04
Immobilized partner concentration in holdup experiment (10-6M): 10
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 3
Protein A protein: FRMPD2
Protein A fragment: 1073-1166
Protein A site: PDZ_3
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: HPV82-E6
Protein B fragment: 142-151
Protein B site: PBM
Protein B sequence: PPRQRSETQV
Protein B construct: biotin-ttds-PPRQRSETQV
Average holdup BI: -0.07
Immobilized partner concentration in holdup experiment (10-6M): 10
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 4
Protein A protein: FRMPD2
Protein A fragment: 770-861
Protein A site: PDZ_1
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: HPV82-E6
Protein B fragment: 142-151
Protein B site: PBM
Protein B sequence: PPRQRSETQV
Protein B construct: biotin-ttds-PPRQRSETQV
Average holdup BI: 0.08
Holdup BI standard deviation: 0.02
Normalized holdup BI: 0.08
Normalized holdup BI standard deviation: 0.02
Immobilized partner concentration in holdup experiment (10-6M): 27.4
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 2
Measured pKd: 3.5
Experiment series 5
Protein A protein: FRMPD2
Protein A fragment: 944-1038
Protein A site: PDZ_2
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: HPV82-E6
Protein B fragment: 142-151
Protein B site: PBM
Protein B sequence: PPRQRSETQV
Protein B construct: biotin-ttds-PPRQRSETQV
Average holdup BI: 0.02
Holdup BI standard deviation: 0.01
Normalized holdup BI: 0.02
Normalized holdup BI standard deviation: 0.01
Immobilized partner concentration in holdup experiment (10-6M): 27.4
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 2
The affinity was below the detection threshold of the assay.
Experiment series 6
Protein A protein: FRMPD2
Protein A fragment: 1073-1166
Protein A site: PDZ_3
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: HPV82-E6
Protein B fragment: 142-151
Protein B site: PBM
Protein B sequence: PPRQRSETQV
Protein B construct: biotin-ttds-PPRQRSETQV
Average holdup BI: 0.03
Holdup BI standard deviation: 0.08
Normalized holdup BI: 0.03
Normalized holdup BI standard deviation: 0.08
Immobilized partner concentration in holdup experiment (10-6M): 27.4
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 2
The affinity was below the detection threshold of the assay.