Details of experiments between Q68DX3 and P27037
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: FRMPD2
Protein A fragment: 770-861
Protein A site: PDZ_1
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: ACVR2A
Protein B fragment: 504-513
Protein B site: PBM
Protein B sequence: VDFPPKESSL
Protein B construct: biotin-ttds-VDFPPKESSL
Average holdup BI: 0.18
Normalized holdup BI: 0.18
Immobilized partner concentration in holdup experiment (10-6M): 33
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 3.82
Experiment series 2
Protein A protein: FRMPD2
Protein A fragment: 944-1038
Protein A site: PDZ_2
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: ACVR2A
Protein B fragment: 504-513
Protein B site: PBM
Protein B sequence: VDFPPKESSL
Protein B construct: biotin-ttds-VDFPPKESSL
Average holdup BI: 0.01
Normalized holdup BI: 0.01
Immobilized partner concentration in holdup experiment (10-6M): 33
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 3
Protein A protein: FRMPD2
Protein A fragment: 1073-1166
Protein A site: PDZ_3
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: ACVR2A
Protein B fragment: 504-513
Protein B site: PBM
Protein B sequence: VDFPPKESSL
Protein B construct: biotin-ttds-VDFPPKESSL
Average holdup BI: -0.02
Normalized holdup BI: -0.02
Immobilized partner concentration in holdup experiment (10-6M): 33
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.