Details of experiments between Q68DX3 and O75096
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: FRMPD2
Protein A fragment: 944-1038
Protein A site: PDZ_2
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: LRP4
Protein B fragment: 1896-1905
Protein B site: PBM
Protein B sequence: ERKLSSESQV
Protein B construct: biotin-ttds-ERKLSSESQV
Average holdup BI: 0.05
Normalized holdup BI: 0.05
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 3.48
Experiment series 2
Protein A protein: FRMPD2
Protein A fragment: 770-861
Protein A site: PDZ_1
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: LRP4
Protein B fragment: 1896-1905
Protein B site: PBM
Protein B sequence: ERKLSSESQV
Protein B construct: biotin-ttds-ERKLSSESQV
Average holdup BI: -0.12
Normalized holdup BI: -0.12
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 3
Protein A protein: FRMPD2
Protein A fragment: 1073-1166
Protein A site: PDZ_3
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: LRP4
Protein B fragment: 1896-1905
Protein B site: PBM
Protein B sequence: ERKLSSESQV
Protein B construct: biotin-ttds-ERKLSSESQV
Average holdup BI: -0.04
Normalized holdup BI: -0.04
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.