Details of experiments between the fragments Q68D42~226-235 and Q8TEW0~582-685
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: TMEM215
Protein A fragment: 226-235
Protein A site: PBM
Protein A sequence: QGRWDHETIV
Protein A construct: Biotin-Ahx-QGRWDHETIV
Protein B protein: PARD3
Protein B fragment: 582-685
Protein B site: PDZ_3
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.33
Normalized holdup BI: 0.33
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 4.47