Details of experiments between Q5VTJ3 and Q02410
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: KLHDC7A
Protein A fragment: 768-777
Protein A site: PBM
Protein A sequence: PTPDLPQTRV
Protein A construct: biotin-ado-ado-PTPDLPQTRV
Protein B protein: APBA1
Protein B fragment: 743-822
Protein B site: PDZ_2
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.05
Normalized holdup BI: 0.06
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Experimental details: Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality causing a systematic error in reported affinities.
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 3.54
Experiment series 2
Protein A protein: KLHDC7A
Protein A fragment: 768-777
Protein A site: PBM
Protein A sequence: PTPDLPQTRV
Protein A construct: biotin-ado-ado-PTPDLPQTRV
Protein B protein: APBA1
Protein B fragment: 655-741
Protein B site: PDZ_1
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: -0
Normalized holdup BI: -0
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Experimental details: Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality causing a systematic error in reported affinities.
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.