Details of experiments between the fragments Q5VST9~5594-5674 and O43516~155-169
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: OBSCN
Protein A fragment: 5594-5674
Protein A site: SH3
Protein A construct: his6-MBP-TEVsite-SH3
Protein B protein: WIPF1
Protein B fragment: 155-169
Protein B site: PxxP_2
Protein B sequence: PGHRSGPPEPQRNRM
Protein B construct: Bio Ado Ado-PGHRSGPPEPQRNRM-CONH2
Average holdup BI: -0.02
Holdup BI standard deviation: 0.01
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/18
Number of measurements: 2
The affinity was below the detection threshold of the assay.