Details of experiments between the fragments Q5TCQ9~572-655 and Q96KB5~313-322
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: MAGI3
Protein A fragment: 572-655
Protein A site: PDZ_3
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: PBK
Protein B fragment: 313-322
Protein B site: PBM
Protein B sequence: HIVEALETDV
Protein B construct: biotin-ttds-HIVEALETDV
Average holdup BI: 0.1
Immobilized partner concentration in holdup experiment (10-6M): 5.5
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 4.33