Details of experiments between Q5TCQ9 and Q14CM0
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: MAGI3
Protein A fragment: 398-503
Protein A site: PDZ_2
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: FRMPD4
Protein B fragment: 1313-1322
Protein B site: PBM
Protein B sequence: RLPKIKETTV
Protein B construct: biotin-ttds-RLPKIKETTV
Average holdup BI: 0.69
Immobilized partner concentration in holdup experiment (10-6M): 6.6
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 5.76
Experiment series 2
Protein A protein: MAGI3
Protein A fragment: 1-114
Protein A site: PDZ_1
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: FRMPD4
Protein B fragment: 1313-1322
Protein B site: PBM
Protein B sequence: RLPKIKETTV
Protein B construct: biotin-ttds-RLPKIKETTV
Average holdup BI: 0.01
Immobilized partner concentration in holdup experiment (10-6M): 6.6
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 3
Protein A protein: MAGI3
Protein A fragment: 572-655
Protein A site: PDZ_3
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: FRMPD4
Protein B fragment: 1313-1322
Protein B site: PBM
Protein B sequence: RLPKIKETTV
Protein B construct: biotin-ttds-RLPKIKETTV
Average holdup BI: 0.06
Immobilized partner concentration in holdup experiment (10-6M): 6.6
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 4
Protein A protein: MAGI3
Protein A fragment: 716-815
Protein A site: PDZ_4
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: FRMPD4
Protein B fragment: 1313-1322
Protein B site: PBM
Protein B sequence: RLPKIKETTV
Protein B construct: biotin-ttds-RLPKIKETTV
Average holdup BI: 0.02
Immobilized partner concentration in holdup experiment (10-6M): 6.6
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 5
Protein A protein: MAGI3
Protein A fragment: 847-941
Protein A site: PDZ_5
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: FRMPD4
Protein B fragment: 1313-1322
Protein B site: PBM
Protein B sequence: RLPKIKETTV
Protein B construct: biotin-ttds-RLPKIKETTV
Average holdup BI: 0
Immobilized partner concentration in holdup experiment (10-6M): 6.6
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 6
Protein A protein: MAGI3
Protein A fragment: 1014-1103
Protein A site: PDZ_6
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: FRMPD4
Protein B fragment: 1313-1322
Protein B site: PBM
Protein B sequence: RLPKIKETTV
Protein B construct: biotin-ttds-RLPKIKETTV
Average holdup BI: 0.04
Immobilized partner concentration in holdup experiment (10-6M): 6.6
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 7
Protein A protein: MAGI3
Protein A fragment: 398-503
Protein A site: PDZ_2
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: FRMPD4
Protein B fragment: 1313-1322
Protein B site: PBM
Protein B sequence: RLPKIKETTV
Protein B construct: biotin-ttds-RLPKIKETTV
Average holdup BI: 0.7
Holdup BI standard deviation: 0
Normalized holdup BI: 0.77
Normalized holdup BI standard deviation: 0
Immobilized partner concentration in holdup experiment (10-6M): 21.6
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 2
Measured pKd: 5.27
Experiment series 8
Protein A protein: MAGI3
Protein A fragment: 398-503
Protein A site: PDZ_2
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: FRMPD4
Protein B fragment: 1313-1322
Protein B site: PBM
Protein B sequence: RLPKIKETTV
Protein B construct: Biotin-ado-ado-RLPKIKETTV
Average holdup BI: 0.83
Normalized holdup BI: 0.88
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Experimental details: Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality. Not reported internal standards of holdup layout #6
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 5.68