Details of experiments between the fragments Q5T5U3~47-159 and Q6IQ26~1278-1287
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: ARHGAP21
Protein A fragment: 47-159
Protein A site: PDZ
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: DENND5A
Protein B fragment: 1278-1287
Protein B site: PBM
Protein B sequence: ETSLVKGIDI
Protein B construct: biotin-ado-ado-ETSLVKGIDI
Average holdup BI: 0.23
Normalized holdup BI: 0.23
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Experimental details: Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality causing a systematic error in reported affinities.
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 4.25