Details of experiments between the fragments Q5T5U3~47-159 and P36809~144-153
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: ARHGAP21
Protein A fragment: 47-159
Protein A site: PDZ
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: HPV30-E6
Protein B fragment: 144-153
Protein B site: PBM
Protein B sequence: RHTTSTETAV
Protein B construct: biotin-ttds-RHTTSTETAV
Average holdup BI: -0.04
Immobilized partner concentration in holdup experiment (10-6M): 4
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.