Details of experiments between the fragments Q5T2W1~510-519 and Q8NI35~547-651
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: NHERF3
Protein A fragment: 510-519
Protein A site: PBM
Protein A sequence: SSSNSEDTEM
Protein A construct: Biotin-Ahx-SSSNSEDTEM
Protein B protein: Inadl
Protein B fragment: 547-651
Protein B site: PDZ_4
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.01
Normalized holdup BI: 0.01
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.