Details of experiments between the fragments Q5T2W1~2-107 and Q9Q6P4~3424-3433
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: NHERF3
Protein A fragment: 2-107
Protein A site: PDZ_1
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: WNV-NS5
Protein B fragment: 3424-3433
Protein B site: PBM
Protein B sequence: DTTLVEDTVL
Protein B construct: biotin-(PEG)3-RYEDTTLVEDTVL
Average holdup BI: 0.4
Holdup BI standard deviation: 0.16
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 4
Measured pKd: 4.61
Experiment series 2
Protein A protein: NHERF3
Protein A fragment: 2-107
Protein A site: PDZ_1
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: WNV-NS5
Protein B fragment: 3424-3433
Protein B site: PBM
Protein B sequence: DTTLVEDTVL
Protein B construct: biotin-(PEG)3-RYEDTTLVEDTVL
Average holdup BI: 0.29
Normalized holdup BI: 0.31
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 4.43