Details of experiments between the fragments Q5T2W1~2-107 and Q68EM7~872-881
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: NHERF3
Protein A fragment: 2-107
Protein A site: PDZ_1
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: ARHGAP17
Protein B fragment: 872-881
Protein B site: PBM
Protein B sequence: IDNDTESTAL
Protein B construct: biotin-ttds-IDNDTESTAL
Average holdup BI: 0.68
Normalized holdup BI: 0.73
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 5.25