Details of experiments between the fragments Q5T2W1~2-107 and P07550~404-413
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: NHERF3
Protein A fragment: 2-107
Protein A site: PDZ_1
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: ADRB2
Protein B fragment: 404-413
Protein B site: PBM
Protein B sequence: RNCSTNDSLL
Protein B construct: Biotin-Ahx-RNCSTNDSLL
Average holdup BI: 0.11
Normalized holdup BI: 0.12
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 3.89