Details of experiments between the fragments Q5T2T1~120-224 and Q9Y597~806-815
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: MPP7
Protein A fragment: 120-224
Protein A site: PDZ
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: KCTD3
Protein B fragment: 806-815
Protein B site: PBM
Protein B sequence: SDSSGQEYSL
Protein B construct: Biotin-Ahx-SDSSGQEYSL
Average holdup BI: 0.18
Normalized holdup BI: 0.18
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 4.1