Details of experiments between Q5SYB0 and Q8NI35
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: FRMPD1
Protein A fragment: 1569-1578
Protein A site: PBM
Protein A sequence: TQKFRASTAL
Protein A construct: Biotin-Ahx-TQKFRASTAL
Protein B protein: Inadl
Protein B fragment: 1673-1763
Protein B site: PDZ_10
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.07
Normalized holdup BI: 0.07
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 3.61
Experiment series 2
Protein A protein: FRMPD1
Protein A fragment: 1569-1578
Protein A site: PBM
Protein A sequence: TQKFRASTAL
Protein A construct: Biotin-Ahx-TQKFRASTAL
Protein B protein: Inadl
Protein B fragment: 113-229
Protein B site: PDZ_1
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.01
Normalized holdup BI: 0.01
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 3
Protein A protein: FRMPD1
Protein A fragment: 1569-1578
Protein A site: PBM
Protein A sequence: TQKFRASTAL
Protein A construct: Biotin-Ahx-TQKFRASTAL
Protein B protein: Inadl
Protein B fragment: 238-335
Protein B site: PDZ_2
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.04
Normalized holdup BI: 0.04
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 4
Protein A protein: FRMPD1
Protein A fragment: 1569-1578
Protein A site: PBM
Protein A sequence: TQKFRASTAL
Protein A construct: Biotin-Ahx-TQKFRASTAL
Protein B protein: Inadl
Protein B fragment: 359-469
Protein B site: PDZ_3
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.04
Normalized holdup BI: 0.05
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 5
Protein A protein: FRMPD1
Protein A fragment: 1569-1578
Protein A site: PBM
Protein A sequence: TQKFRASTAL
Protein A construct: Biotin-Ahx-TQKFRASTAL
Protein B protein: Inadl
Protein B fragment: 547-651
Protein B site: PDZ_4
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0
Normalized holdup BI: 0
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 6
Protein A protein: FRMPD1
Protein A fragment: 1569-1578
Protein A site: PBM
Protein A sequence: TQKFRASTAL
Protein A construct: Biotin-Ahx-TQKFRASTAL
Protein B protein: Inadl
Protein B fragment: 673-780
Protein B site: PDZ_5
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.01
Normalized holdup BI: 0.01
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 7
Protein A protein: FRMPD1
Protein A fragment: 1569-1578
Protein A site: PBM
Protein A sequence: TQKFRASTAL
Protein A construct: Biotin-Ahx-TQKFRASTAL
Protein B protein: Inadl
Protein B fragment: 1058-1167
Protein B site: PDZ_6
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.02
Normalized holdup BI: 0.02
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 8
Protein A protein: FRMPD1
Protein A fragment: 1569-1578
Protein A site: PBM
Protein A sequence: TQKFRASTAL
Protein A construct: Biotin-Ahx-TQKFRASTAL
Protein B protein: Inadl
Protein B fragment: 1425-1530
Protein B site: PDZ_8
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.03
Normalized holdup BI: 0.04
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 9
Protein A protein: FRMPD1
Protein A fragment: 1569-1578
Protein A site: PBM
Protein A sequence: TQKFRASTAL
Protein A construct: Biotin-Ahx-TQKFRASTAL
Protein B protein: Inadl
Protein B fragment: 1530-1615
Protein B site: PDZ_9
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.02
Normalized holdup BI: 0.02
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 10
Protein A protein: FRMPD1
Protein A fragment: 1569-1578
Protein A site: PBM
Protein A sequence: TQKFRASTAL
Protein A construct: Biotin-Ahx-TQKFRASTAL
Protein B protein: InaDl
Protein B fragment: 1425-1530
Protein B site: PDZ_8
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.02
Normalized holdup BI: 0.02
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Experimental details: not reported internal standards of holdup layout #6
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 11
Protein A protein: FRMPD1
Protein A fragment: 1569-1578
Protein A site: PBM
Protein A sequence: TQKFRASTAL
Protein A construct: Biotin-Ahx-TQKFRASTAL
Protein B protein: InaDl
Protein B fragment: 673-780
Protein B site: PDZ_5
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: -0.01
Normalized holdup BI: -0.01
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Experimental details: not reported internal standards of holdup layout #6
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 12
Protein A protein: FRMPD1
Protein A fragment: 1569-1578
Protein A site: PBM
Protein A sequence: TQKFRASTAL
Protein A construct: Biotin-Ahx-TQKFRASTAL
Protein B protein: InaDl
Protein B fragment: 359-469
Protein B site: PDZ_3
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: -0
Normalized holdup BI: -0
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Experimental details: not reported internal standards of holdup layout #6
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 13
Protein A protein: FRMPD1
Protein A fragment: 1569-1578
Protein A site: PBM
Protein A sequence: TQKFRASTAL
Protein A construct: Biotin-Ahx-TQKFRASTAL
Protein B protein: InaDl
Protein B fragment: 1673-1763
Protein B site: PDZ_10
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0
Normalized holdup BI: 0
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Experimental details: not reported internal standards of holdup layout #6
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 14
Protein A protein: FRMPD1
Protein A fragment: 1569-1578
Protein A site: PBM
Protein A sequence: TQKFRASTAL
Protein A construct: Biotin-Ahx-TQKFRASTAL
Protein B protein: InaDl
Protein B fragment: 1530-1615
Protein B site: PDZ_9
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: -0
Normalized holdup BI: -0
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Experimental details: not reported internal standards of holdup layout #6
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 15
Protein A protein: FRMPD1
Protein A fragment: 1569-1578
Protein A site: PBM
Protein A sequence: TQKFRASTAL
Protein A construct: Biotin-Ahx-TQKFRASTAL
Protein B protein: InaDl
Protein B fragment: 1058-1167
Protein B site: PDZ_6
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: -0.02
Normalized holdup BI: -0.02
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Experimental details: not reported internal standards of holdup layout #6
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 16
Protein A protein: FRMPD1
Protein A fragment: 1569-1578
Protein A site: PBM
Protein A sequence: TQKFRASTAL
Protein A construct: Biotin-Ahx-TQKFRASTAL
Protein B protein: InaDl
Protein B fragment: 547-651
Protein B site: PDZ_4
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: -0.02
Normalized holdup BI: -0.02
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Experimental details: not reported internal standards of holdup layout #6
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 17
Protein A protein: FRMPD1
Protein A fragment: 1569-1578
Protein A site: PBM
Protein A sequence: TQKFRASTAL
Protein A construct: Biotin-Ahx-TQKFRASTAL
Protein B protein: InaDl
Protein B fragment: 238-335
Protein B site: PDZ_2
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: -0.01
Normalized holdup BI: -0.01
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Experimental details: not reported internal standards of holdup layout #6
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 18
Protein A protein: FRMPD1
Protein A fragment: 1569-1578
Protein A site: PBM
Protein A sequence: TQKFRASTAL
Protein A construct: Biotin-Ahx-TQKFRASTAL
Protein B protein: InaDl
Protein B fragment: 113-229
Protein B site: PDZ_1
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: -0.02
Normalized holdup BI: -0.02
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Experimental details: not reported internal standards of holdup layout #6
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.