Details of experiments between Q5SYB0 and Q13424
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: FRMPD1
Protein A fragment: 1569-1578
Protein A site: PBM
Protein A sequence: TQKFRASTAL
Protein A construct: Biotin-Ahx-TQKFRASTAL
Protein B protein: SNTA1
Protein B fragment: 84-171
Protein B site: PDZ
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.03
Normalized holdup BI: 0.04
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 2
Protein A protein: FRMPD1
Protein A fragment: 1569-1578
Protein A site: PBM
Protein A sequence: TQKFRASTAL
Protein A construct: Biotin-Ahx-TQKFRASTAL
Protein B protein: SNTA1
Protein B fragment: 84-171
Protein B site: PDZ
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: -0.01
Normalized holdup BI: -0.01
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Experimental details: not reported internal standards of holdup layout #6
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.