Details of experiments between Q16513 and Q15700
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: PKN2
Protein A fragment: 975-984
Protein A site: PBM
Protein A sequence: RDFDYIADWC
Protein A construct: biotin-ado-ado-RDFDYIADWC
Protein B protein: DLG2
Protein B fragment: 418-513
Protein B site: PDZ_3
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.09
Normalized holdup BI: 0.09
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Experimental details: Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality causing a systematic error in reported affinities.
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 3.77
Experiment series 2
Protein A protein: PKN2
Protein A fragment: 975-984
Protein A site: PBM
Protein A sequence: RDFDYIADWC
Protein A construct: biotin-ado-ado-RDFDYIADWC
Protein B protein: DLG2
Protein B fragment: 189-283
Protein B site: PDZ_2
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: -0.09
Normalized holdup BI: -0.11
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Experimental details: Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality causing a systematic error in reported affinities.
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.