Details of experiments between Q15700 and Q8WWN8
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: DLG2
Protein A fragment: 418-513
Protein A site: PDZ_3
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: ARAP3
Protein B fragment: 1417-1431
Protein B site: Internal_PBM
Protein B construct: Biotin-GSGSGS-ELIQDTSTSFSTTREW-CONH2
Average holdup BI: 0.08
Normalized holdup BI: 0.08
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 3.69
Experiment series 2
Protein A protein: DLG2
Protein A fragment: 189-283
Protein A site: PDZ_2
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: ARAP3
Protein B fragment: 1417-1431
Protein B site: Internal_PBM
Protein B construct: Biotin-GSGSGS-ELIQDTSTSFSTTREW-CONH2
Average holdup BI: -0
Normalized holdup BI: -0
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.