Details of experiments between Q15700 and O00192
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: DLG2
Protein A fragment: 418-513
Protein A site: PDZ_3
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: ARVCF
Protein B fragment: 953-962
Protein B site: PBM
Protein B sequence: AKPQPVDSWV
Protein B construct: Biotin-Ahx-AKPQPVDSWV
Average holdup BI: 0.17
Normalized holdup BI: 0.17
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 4.06
Experiment series 2
Protein A protein: DLG2
Protein A fragment: 189-283
Protein A site: PDZ_2
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: ARVCF
Protein B fragment: 953-962
Protein B site: PBM
Protein B sequence: AKPQPVDSWV
Protein B construct: Biotin-Ahx-AKPQPVDSWV
Average holdup BI: -0
Normalized holdup BI: -0
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.