Details of experiments between the fragments Q15418~683-735 and P57105~6-103
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: RPS6KA1
Protein A fragment: 683-735
Protein A site: PBM
Protein A sequence: RVRKLPSTTL
Protein A construct: ...KLPSTTL
Protein B protein: SYNJ2BP
Protein B fragment: 6-103
Protein B site: PDZ
Protein B construct: his6-MBP-TEVsite-PDZ
Measured dissociation constant (10-6M): 0
Experiment method: FP_competitive
PUBMED ID of publication: 30726710
Number of measurements: 3
The affinity was below the detection threshold of the assay.
Experiment series 2
Protein A protein: RPS6KA1
Protein A fragment: 683-735
Protein A site: PBM
Protein A modification: (SEP)732
Protein A sequence: RVRKLPSTTL
Protein A construct: ...KLP(pS)TTL
Protein B protein: SYNJ2BP
Protein B fragment: 6-103
Protein B site: PDZ
Protein B construct: his6-MBP-TEVsite-PDZ
Measured dissociation constant (10-6M): 7
Standard deviation of dissociation constant (10-6M): 1
Experiment method: FP_competitive
PUBMED ID of publication: 30726710
Number of measurements: 3
Measured pKd: 5.15
Experiment series 3
Protein A protein: RPS6KA1
Protein A fragment: 683-735
Protein A site: PBM
Protein A sequence: RVRKLPSTTL
Protein A construct: ...KLPSTTL
Protein B protein: SYNJ2BP
Protein B fragment: 6-103
Protein B site: PDZ
Protein B construct: his6-MBP-TEVsite-PDZ
Measured dissociation constant (10-6M): 51
Experiment method: ITC
PUBMED ID of publication: 30726710
Number of measurements: 1
Measured pKd: 4.29
Experiment series 4
Protein A protein: RPS6KA1
Protein A fragment: 683-735
Protein A site: PBM
Protein A modification: (SEP)732
Protein A sequence: RVRKLPSTTL
Protein A construct: ...KLP(pS)TTL
Protein B protein: SYNJ2BP
Protein B fragment: 6-103
Protein B site: PDZ
Protein B construct: his6-MBP-TEVsite-PDZ
Measured dissociation constant (10-6M): 19
Experiment method: ITC
PUBMED ID of publication: 30726710
Number of measurements: 1
Measured pKd: 4.72