Details of experiments between the fragments Q15311~646-655 and P78352~306-403
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: RALBP1
Protein A fragment: 646-655
Protein A site: PBM
Protein A sequence: PSRDRKETSI
Protein A construct: biotin-ttds-PSRDRKETSI
Protein B protein: DLG4
Protein B fragment: 306-403
Protein B site: PDZ_3
Protein B construct: his6-MBP-TEVsite-PDZ
Measured dissociation constant (10-6M): 10.2
Standard deviation of dissociation constant (10-6M): 0.81
Experiment method: competitive FP
Details of affinity fitting: Competitive binding equation in ProFit
PUBMED ID of publication: 36115835
Number of measurements: 3
Measured pKd: 4.99
Experiment series 2
Protein A protein: RALBP1
Protein A fragment: 646-655
Protein A site: PBM
Protein A sequence: PSRDRKETSI
Protein A construct: biotin-ttds-PSRDRKETSI
Protein B protein: DLG4
Protein B fragment: 306-403
Protein B site: PDZ_3
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.27
Immobilized partner concentration in holdup experiment (10-6M): 20
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 4.3
Experiment series 3
Protein A protein: RALBP1
Protein A fragment: 646-655
Protein A site: PBM
Protein A sequence: PSRDRKETSI
Protein A construct: biotin-ttds-PSRDRKETSI
Protein B protein: DLG4
Protein B fragment: 306-403
Protein B site: PDZ_3
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.57
Holdup BI standard deviation: 0.1
Normalized holdup BI: 0.65
Normalized holdup BI standard deviation: 0.11
Immobilized partner concentration in holdup experiment (10-6M): 28
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 2
Measured pKd: 4.87
Experiment series 4
Protein A protein: RALBP1
Protein A fragment: 646-655
Protein A site: PBM
Protein A sequence: PSRDRKETSI
Protein A construct: biotin-ttds-PSRDRKETSI
Protein B protein: DLG4
Protein B fragment: 306-403
Protein B site: PDZ_3
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.61
Normalized holdup BI: 0.72
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Experimental details: not reported internal standards of holdup layout #6
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 5.23