Details of experiments between Q15311 and Q9C0E4
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: RALBP1
Protein A fragment: 646-655
Protein A site: PBM
Protein A sequence: PSRDRKETSI
Protein A construct: biotin-ttds-PSRDRKETSI
Protein B protein: GRIP2
Protein B fragment: 137-245
Protein B site: PDZ_2
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.25
Immobilized partner concentration in holdup experiment (10-6M): 20
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 4.25
Experiment series 2
Protein A protein: RALBP1
Protein A fragment: 646-655
Protein A site: PBM
Protein A sequence: PSRDRKETSI
Protein A construct: biotin-ttds-PSRDRKETSI
Protein B protein: GRIP2
Protein B fragment: 238-339
Protein B site: PDZ_3
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.12
Immobilized partner concentration in holdup experiment (10-6M): 20
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 3.85
Experiment series 3
Protein A protein: RALBP1
Protein A fragment: 646-655
Protein A site: PBM
Protein A sequence: PSRDRKETSI
Protein A construct: biotin-ttds-PSRDRKETSI
Protein B protein: GRIP2
Protein B fragment: 41-133
Protein B site: PDZ_1
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.01
Immobilized partner concentration in holdup experiment (10-6M): 20
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 4
Protein A protein: RALBP1
Protein A fragment: 646-655
Protein A site: PBM
Protein A sequence: PSRDRKETSI
Protein A construct: biotin-ttds-PSRDRKETSI
Protein B protein: GRIP2
Protein B fragment: 446-548
Protein B site: PDZ_4
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.03
Immobilized partner concentration in holdup experiment (10-6M): 20
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 5
Protein A protein: RALBP1
Protein A fragment: 646-655
Protein A site: PBM
Protein A sequence: PSRDRKETSI
Protein A construct: biotin-ttds-PSRDRKETSI
Protein B protein: GRIP2
Protein B fragment: 544-642
Protein B site: PDZ_5
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.05
Immobilized partner concentration in holdup experiment (10-6M): 20
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 6
Protein A protein: RALBP1
Protein A fragment: 646-655
Protein A site: PBM
Protein A sequence: PSRDRKETSI
Protein A construct: biotin-ttds-PSRDRKETSI
Protein B protein: GRIP2
Protein B fragment: 649-740
Protein B site: PDZ_6
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0
Immobilized partner concentration in holdup experiment (10-6M): 20
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 7
Protein A protein: RALBP1
Protein A fragment: 646-655
Protein A site: PBM
Protein A sequence: PSRDRKETSI
Protein A construct: biotin-ttds-PSRDRKETSI
Protein B protein: GRIP2
Protein B fragment: 934-1023
Protein B site: PDZ_7
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: -0.03
Immobilized partner concentration in holdup experiment (10-6M): 20
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 8
Protein A protein: RALBP1
Protein A fragment: 646-655
Protein A site: PBM
Protein A sequence: PSRDRKETSI
Protein A construct: biotin-ttds-PSRDRKETSI
Protein B protein: GRIP2
Protein B fragment: 649-740
Protein B site: PDZ_6
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.06
Normalized holdup BI: 0.06
Immobilized partner concentration in holdup experiment (10-6M): 28
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 3.36
Experiment series 9
Protein A protein: RALBP1
Protein A fragment: 646-655
Protein A site: PBM
Protein A sequence: PSRDRKETSI
Protein A construct: biotin-ttds-PSRDRKETSI
Protein B protein: GRIP2
Protein B fragment: 238-339
Protein B site: PDZ_3
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0
Normalized holdup BI: 0
Immobilized partner concentration in holdup experiment (10-6M): 28
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 10
Protein A protein: RALBP1
Protein A fragment: 646-655
Protein A site: PBM
Protein A sequence: PSRDRKETSI
Protein A construct: biotin-ttds-PSRDRKETSI
Protein B protein: GRIP2
Protein B fragment: 446-548
Protein B site: PDZ_4
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0
Normalized holdup BI: 0
Immobilized partner concentration in holdup experiment (10-6M): 28
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 11
Protein A protein: RALBP1
Protein A fragment: 646-655
Protein A site: PBM
Protein A sequence: PSRDRKETSI
Protein A construct: biotin-ttds-PSRDRKETSI
Protein B protein: GRIP2
Protein B fragment: 544-642
Protein B site: PDZ_5
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.01
Normalized holdup BI: 0.01
Immobilized partner concentration in holdup experiment (10-6M): 28
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 12
Protein A protein: RALBP1
Protein A fragment: 646-655
Protein A site: PBM
Protein A sequence: PSRDRKETSI
Protein A construct: biotin-ttds-PSRDRKETSI
Protein B protein: GRIP2
Protein B fragment: 934-1023
Protein B site: PDZ_7
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: -0.16
Normalized holdup BI: -0.16
Immobilized partner concentration in holdup experiment (10-6M): 28
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 13
Protein A protein: RALBP1
Protein A fragment: 646-655
Protein A site: PBM
Protein A sequence: PSRDRKETSI
Protein A construct: biotin-ttds-PSRDRKETSI
Protein B protein: GRIP2
Protein B fragment: 649-740
Protein B site: PDZ_6
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: -0.01
Normalized holdup BI: -0.01
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Experimental details: not reported internal standards of holdup layout #6
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 14
Protein A protein: RALBP1
Protein A fragment: 646-655
Protein A site: PBM
Protein A sequence: PSRDRKETSI
Protein A construct: biotin-ttds-PSRDRKETSI
Protein B protein: GRIP2
Protein B fragment: 544-642
Protein B site: PDZ_5
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: -0.03
Normalized holdup BI: -0.03
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Experimental details: not reported internal standards of holdup layout #6
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 15
Protein A protein: RALBP1
Protein A fragment: 646-655
Protein A site: PBM
Protein A sequence: PSRDRKETSI
Protein A construct: biotin-ttds-PSRDRKETSI
Protein B protein: GRIP2
Protein B fragment: 238-339
Protein B site: PDZ_3
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: -0.03
Normalized holdup BI: -0.03
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Experimental details: not reported internal standards of holdup layout #6
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 16
Protein A protein: RALBP1
Protein A fragment: 646-655
Protein A site: PBM
Protein A sequence: PSRDRKETSI
Protein A construct: biotin-ttds-PSRDRKETSI
Protein B protein: GRIP2
Protein B fragment: 934-1023
Protein B site: PDZ_7
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0
Normalized holdup BI: 0
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Experimental details: not reported internal standards of holdup layout #6
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 17
Protein A protein: RALBP1
Protein A fragment: 646-655
Protein A site: PBM
Protein A sequence: PSRDRKETSI
Protein A construct: biotin-ttds-PSRDRKETSI
Protein B protein: GRIP2
Protein B fragment: 446-548
Protein B site: PDZ_4
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: -0.04
Normalized holdup BI: -0.04
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Experimental details: not reported internal standards of holdup layout #6
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.