Details of experiments between Q15303 and O75970
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: ERBB4
Protein A fragment: 1299-1308
Protein A site: PBM
Protein A sequence: PPYRHRNTVV
Protein A construct: biotin-ado-ado-PPYRHRNTVV
Protein B protein: MPDZ
Protein B fragment: 1624-1716
Protein B site: PDZ_10
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.14
Normalized holdup BI: 0.14
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Experimental details: Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality causing a systematic error in reported affinities.
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 3.95
Experiment series 2
Protein A protein: ERBB4
Protein A fragment: 1299-1308
Protein A site: PBM
Protein A sequence: PPYRHRNTVV
Protein A construct: biotin-ado-ado-PPYRHRNTVV
Protein B protein: MPDZ
Protein B fragment: 1858-1949
Protein B site: PDZ_12
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: -0.02
Normalized holdup BI: -0.02
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Experimental details: Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality causing a systematic error in reported affinities.
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 3
Protein A protein: ERBB4
Protein A fragment: 1299-1308
Protein A site: PBM
Protein A sequence: PPYRHRNTVV
Protein A construct: biotin-ado-ado-PPYRHRNTVV
Protein B protein: MPDZ
Protein B fragment: 688-793
Protein B site: PDZ_5
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.04
Normalized holdup BI: 0.04
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Experimental details: Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality causing a systematic error in reported affinities.
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.