Details of experiments between the fragments Q14CM0~67-160 and Q9Y597~806-815
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: FRMPD4
Protein A fragment: 67-160
Protein A site: PDZ
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: KCTD3
Protein B fragment: 806-815
Protein B site: PBM
Protein B sequence: SDSSGQEYSL
Protein B construct: Biotin-Ahx-SDSSGQEYSL
Average holdup BI: 0.39
Holdup BI standard deviation: 0.04
Normalized holdup BI: 0.45
Normalized holdup BI standard deviation: 0.05
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 2
Measured pKd: 4.69