Details of experiments between Q14CM0 and Q9NR12
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: FRMPD4
Protein A fragment: 1313-1322
Protein A site: PBM
Protein A sequence: RLPKIKETTV
Protein A construct: biotin-ttds-RLPKIKETTV
Protein B protein: PDLIM7
Protein B fragment: 1-85
Protein B site: PDZ
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: -0.01
Immobilized partner concentration in holdup experiment (10-6M): 6.6
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 2
Protein A protein: FRMPD4
Protein A fragment: 67-160
Protein A site: PDZ
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: PDLIM7
Protein B fragment: 448-457
Protein B site: PBM
Protein B sequence: LCKSHAFSHV
Protein B construct: Biotin-Ahx-LCKSHAFSHV
Average holdup BI: -0.02
Holdup BI standard deviation: 0.05
Normalized holdup BI: -0.02
Normalized holdup BI standard deviation: 0.06
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 2
The affinity was below the detection threshold of the assay.