Details of experiments between the fragments Q14721~849-858 and O43464~355-458
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: KCNB1
Protein A fragment: 849-858
Protein A site: PBM
Protein A sequence: AHGSTRDQSI
Protein A construct: biotin-ado-ado-AHGSTRDQSI
Protein B protein: HTRA2
Protein B fragment: 355-458
Protein B site: PDZ
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: -0.01
Normalized holdup BI: -0.01
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Experimental details: Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality causing a systematic error in reported affinities.
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.