Details of experiments between the fragments Q14160~718-814 and Q81964~151-160
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: SCRIB
Protein A fragment: 718-814
Protein A site: PDZ_1
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: HPV59-E6
Protein B fragment: 151-160
Protein B site: PBM
Protein B sequence: QRQARSETLV
Protein B construct: biotin-ttds-QRQARSETLV
Measured dissociation constant (10-6M): 39.22
Standard deviation of dissociation constant (10-6M): 1.79
Experiment method: competitive FP
Details of affinity fitting: Competitive binding equation in ProFit
PUBMED ID of publication: 36115835
Number of measurements: 3
Measured pKd: 4.41
Experiment series 2
Protein A protein: SCRIB
Protein A fragment: 718-814
Protein A site: PDZ_1
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: HPV59-E6
Protein B fragment: 151-160
Protein B site: PBM
Protein B sequence: QRQARSETLV
Protein B construct: biotin-ttds-QRQARSETLV
Average holdup BI: 0.05
Immobilized partner concentration in holdup experiment (10-6M): 7
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 3
Protein A protein: SCRIB
Protein A fragment: 718-814
Protein A site: PDZ_1
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: HPV59-E6
Protein B fragment: 151-160
Protein B site: PBM
Protein B sequence: QRQARSETLV
Protein B construct: biotin-ttds-QRQARSETLV
Average holdup BI: 0.08
Holdup BI standard deviation: 0.06
Normalized holdup BI: 0.08
Normalized holdup BI standard deviation: 0.06
Immobilized partner concentration in holdup experiment (10-6M): 13.7
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 8
Measured pKd: 3.82