Details of experiments between Q14160 and Q9BSE5
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: SCRIB
Protein A fragment: 718-814
Protein A site: PDZ_1
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: AGMAT
Protein B fragment: 343-352
Protein B site: PBM
Protein B sequence: LCALPKVTTV
Protein B construct: biotin-ttds-LCALPKVTTV
Average holdup BI: 0.28
Normalized holdup BI: 0.29
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 4.39
Experiment series 2
Protein A protein: SCRIB
Protein A fragment: 999-1093
Protein A site: PDZ_3
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: AGMAT
Protein B fragment: 343-352
Protein B site: PBM
Protein B sequence: LCALPKVTTV
Protein B construct: biotin-ttds-LCALPKVTTV
Average holdup BI: 0.21
Normalized holdup BI: 0.23
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 4.24
Experiment series 3
Protein A protein: SCRIB
Protein A fragment: 858-949
Protein A site: PDZ_2
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: AGMAT
Protein B fragment: 343-352
Protein B site: PBM
Protein B sequence: LCALPKVTTV
Protein B construct: biotin-ttds-LCALPKVTTV
Average holdup BI: 0.02
Holdup BI standard deviation: 0.05
Normalized holdup BI: 0.03
Normalized holdup BI standard deviation: 0.07
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 2
The affinity was below the detection threshold of the assay.