Details of experiments between Q14160 and P36809
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: SCRIB
Protein A fragment: 718-814
Protein A site: PDZ_1
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: HPV30-E6
Protein B fragment: 144-153
Protein B site: PBM
Protein B sequence: RHTTSTETAV
Protein B construct: biotin-ttds-RHTTSTETAV
Measured dissociation constant (10-6M): 19.68
Standard deviation of dissociation constant (10-6M): 1.2
Experiment method: competitive FP
Details of affinity fitting: Competitive binding equation in ProFit
PUBMED ID of publication: 36115835
Number of measurements: 3
Measured pKd: 4.71
Experiment series 2
Protein A protein: SCRIB
Protein A fragment: 999-1093
Protein A site: PDZ_3
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: HPV30-E6
Protein B fragment: 144-153
Protein B site: PBM
Protein B sequence: RHTTSTETAV
Protein B construct: biotin-ttds-RHTTSTETAV
Average holdup BI: 0.25
Immobilized partner concentration in holdup experiment (10-6M): 4
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 5.06
Experiment series 3
Protein A protein: SCRIB
Protein A fragment: 718-814
Protein A site: PDZ_1
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: HPV30-E6
Protein B fragment: 144-153
Protein B site: PBM
Protein B sequence: RHTTSTETAV
Protein B construct: biotin-ttds-RHTTSTETAV
Average holdup BI: 0.15
Immobilized partner concentration in holdup experiment (10-6M): 4
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 4.7
Experiment series 4
Protein A protein: SCRIB
Protein A fragment: 1090-1197
Protein A site: PDZ_4
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: HPV30-E6
Protein B fragment: 144-153
Protein B site: PBM
Protein B sequence: RHTTSTETAV
Protein B construct: biotin-ttds-RHTTSTETAV
Average holdup BI: 0.12
Immobilized partner concentration in holdup experiment (10-6M): 4
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 4.59
Experiment series 5
Protein A protein: SCRIB
Protein A fragment: 858-949
Protein A site: PDZ_2
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: HPV30-E6
Protein B fragment: 144-153
Protein B site: PBM
Protein B sequence: RHTTSTETAV
Protein B construct: biotin-ttds-RHTTSTETAV
Average holdup BI: 0.1
Immobilized partner concentration in holdup experiment (10-6M): 4
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 4.49
Experiment series 6
Protein A protein: SCRIB
Protein A fragment: 858-949
Protein A site: PDZ_2
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: HPV30-E6
Protein B fragment: 144-153
Protein B site: PBM
Protein B sequence: RHTTSTETAV
Protein B construct: biotin-ttds-RHTTSTETAV
Average holdup BI: 0.47
Normalized holdup BI: 0.59
Immobilized partner concentration in holdup experiment (10-6M): 13.4
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 5.11
Experiment series 7
Protein A protein: SCRIB
Protein A fragment: 999-1093
Protein A site: PDZ_3
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: HPV30-E6
Protein B fragment: 144-153
Protein B site: PBM
Protein B sequence: RHTTSTETAV
Protein B construct: biotin-ttds-RHTTSTETAV
Average holdup BI: 0.49
Normalized holdup BI: 0.54
Immobilized partner concentration in holdup experiment (10-6M): 13.4
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 5.02
Experiment series 8
Protein A protein: SCRIB
Protein A fragment: 718-814
Protein A site: PDZ_1
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: HPV30-E6
Protein B fragment: 144-153
Protein B site: PBM
Protein B sequence: RHTTSTETAV
Protein B construct: biotin-ttds-RHTTSTETAV
Average holdup BI: 0.39
Holdup BI standard deviation: 0.03
Normalized holdup BI: 0.41
Normalized holdup BI standard deviation: 0.03
Immobilized partner concentration in holdup experiment (10-6M): 13.4
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 3
Measured pKd: 4.76