Details of experiments between Q14160 and P17386
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: SCRIB
Protein A fragment: 718-814
Protein A site: PDZ_1
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: HPV31-E6
Protein B fragment: 140-149
Protein B site: PBM
Protein B sequence: SRRPRTETQV
Protein B construct: biotin-ttds-SRRPRTETQV
Average holdup BI: 0.37
Holdup BI standard deviation: 0
Normalized holdup BI: 0.39
Normalized holdup BI standard deviation: 0
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 2
Measured pKd: 4.6
Experiment series 2
Protein A protein: SCRIB
Protein A fragment: 999-1093
Protein A site: PDZ_3
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: HPV31-E6
Protein B fragment: 140-149
Protein B site: PBM
Protein B sequence: SRRPRTETQV
Protein B construct: biotin-ttds-SRRPRTETQV
Average holdup BI: 0.34
Holdup BI standard deviation: 0
Normalized holdup BI: 0.37
Normalized holdup BI standard deviation: 0
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 2
Measured pKd: 4.56
Experiment series 3
Protein A protein: SCRIB
Protein A fragment: 858-949
Protein A site: PDZ_2
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: HPV31-E6
Protein B fragment: 140-149
Protein B site: PBM
Protein B sequence: SRRPRTETQV
Protein B construct: biotin-ttds-SRRPRTETQV
Average holdup BI: 0.23
Holdup BI standard deviation: 0.03
Normalized holdup BI: 0.29
Normalized holdup BI standard deviation: 0.04
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 4
Measured pKd: 4.38