Details of experiments between Q14160 and O00192
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: SCRIB
Protein A fragment: 858-949
Protein A site: PDZ_2
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: ARVCF
Protein B fragment: 953-962
Protein B site: PBM
Protein B sequence: AKPQPVDSWV
Protein B construct: Biotin-Ahx-AKPQPVDSWV
Average holdup BI: 0.53
Normalized holdup BI: 0.69
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 5.16
Experiment series 2
Protein A protein: SCRIB
Protein A fragment: 999-1093
Protein A site: PDZ_3
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: ARVCF
Protein B fragment: 953-962
Protein B site: PBM
Protein B sequence: AKPQPVDSWV
Protein B construct: Biotin-Ahx-AKPQPVDSWV
Average holdup BI: 0.11
Normalized holdup BI: 0.14
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 3.95
Experiment series 3
Protein A protein: SCRIB
Protein A fragment: 718-814
Protein A site: PDZ_1
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: ARVCF
Protein B fragment: 953-962
Protein B site: PBM
Protein B sequence: AKPQPVDSWV
Protein B construct: Biotin-Ahx-AKPQPVDSWV
Average holdup BI: 0.07
Holdup BI standard deviation: 0.01
Normalized holdup BI: 0.09
Normalized holdup BI standard deviation: 0.01
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 2
Measured pKd: 3.74