Details of experiments between the fragments Q14155~794-803 and Q9Y3R0~242-344
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: ARHGEF7
Protein A fragment: 794-803
Protein A site: PBM
Protein A sequence: NDPAWDETNL
Protein A construct: biotin-ttds-NDPAWDETNL
Protein B protein: GRIP1
Protein B fragment: 242-344
Protein B site: PDZ_3
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.02
Holdup BI standard deviation: 0.03
Normalized holdup BI: 0.02
Normalized holdup BI standard deviation: 0.03
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 2
The affinity was below the detection threshold of the assay.