Details of experiments between the fragments Q14155~178-251 and Q8ND82~92-106
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: ARHGEF7
Protein A fragment: 178-251
Protein A site: SH3
Protein A construct: his6-MBP-TEVsite-SH3
Protein B protein: ZNF280C
Protein B fragment: 92-106
Protein B site: PxxP_2
Protein B sequence: QRCRDPPSNPVAASP
Protein B construct: Bio Ado Ado-QRCRDPPSNPVAASP-CONH2
Average holdup BI: 0.02
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/17
Number of measurements: 1
The affinity was below the detection threshold of the assay.