Details of experiments between the fragments Q14155~178-251 and Q6P1N0~3-17
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: ARHGEF7
Protein A fragment: 178-251
Protein A site: SH3
Protein A construct: his6-MBP-TEVsite-SH3
Protein B protein: CC2D1A
Protein B fragment: 3-17
Protein B site: PxxP_1
Protein B sequence: KRKGPPGPPGRGAAA
Protein B construct: Bio Ado Ado-KRKGPPGPPGRGAAA-CONH2
Average holdup BI: 0.01
Holdup BI standard deviation: 0.02
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/17
Number of measurements: 3
The affinity was below the detection threshold of the assay.