Details of experiments between the fragments Q14155~178-251 and O43516~260-274
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: ARHGEF7
Protein A fragment: 178-251
Protein A site: SH3
Protein A construct: his6-MBP-TEVsite-SH3
Protein B protein: WIPF1
Protein B fragment: 260-274
Protein B site: PxxP_6
Protein B sequence: LDDKPPPPPPPVGNR
Protein B construct: Bio Ado Ado-LDDKPPPPPPPVGNR-CONH2
Average holdup BI: 0.08
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/17
Number of measurements: 1
Measured pKd: 3.71