Details of experiments between Q14155 and Q9NZN5
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: ARHGEF7
Protein A fragment: 794-803
Protein A site: PBM
Protein A sequence: NDPAWDETNL
Protein A construct: biotin-ttds-NDPAWDETNL
Protein B protein: ARHGEF12
Protein B fragment: 67-151
Protein B site: PDZ
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.14
Holdup BI standard deviation: 0.07
Normalized holdup BI: 0.16
Normalized holdup BI standard deviation: 0.07
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 2
Measured pKd: 4.03