Details of experiments between Q14155 and Q96QZ7
Note that the results of all experiments are listed, regardless of the modification states of the fragments.



Experiment series 1

Protein A protein: ARHGEF7
Protein A fragment: 794-803
Protein A site: PBM
Protein A sequence: NDPAWDETNL
Protein A construct: biotin-ttds-NDPAWDETNL

Protein B protein: MAGI1
Protein B fragment: 1-106
Protein B site: PDZ_1
Protein B construct: his6-MBP-TEVsite-PDZ

Average holdup BI: -0.06
Holdup BI standard deviation: 0.05
Normalized holdup BI: -0.06
Normalized holdup BI standard deviation: 0.05
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 2
The affinity was below the detection threshold of the assay.


Experiment series 2

Protein A protein: ARHGEF7
Protein A fragment: 794-803
Protein A site: PBM
Protein A sequence: NDPAWDETNL
Protein A construct: biotin-ttds-NDPAWDETNL

Protein B protein: MAGI1
Protein B fragment: 456-580
Protein B site: PDZ_2
Protein B construct: his6-MBP-TEVsite-PDZ

Average holdup BI: 0.02
Holdup BI standard deviation: 0.03
Normalized holdup BI: 0.02
Normalized holdup BI standard deviation: 0.04
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 2
The affinity was below the detection threshold of the assay.