Details of experiments between Q14155 and Q8NI35
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: ARHGEF7
Protein A fragment: 794-803
Protein A site: PBM
Protein A sequence: NDPAWDETNL
Protein A construct: biotin-ttds-NDPAWDETNL
Protein B protein: Inadl
Protein B fragment: 1058-1167
Protein B site: PDZ_6
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.16
Holdup BI standard deviation: 0.02
Normalized holdup BI: 0.18
Normalized holdup BI standard deviation: 0.02
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 4
Measured pKd: 4.09
Experiment series 2
Protein A protein: ARHGEF7
Protein A fragment: 794-803
Protein A site: PBM
Protein A sequence: NDPAWDETNL
Protein A construct: biotin-ttds-NDPAWDETNL
Protein B protein: Inadl
Protein B fragment: 113-229
Protein B site: PDZ_1
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.05
Holdup BI standard deviation: 0.04
Normalized holdup BI: 0.05
Normalized holdup BI standard deviation: 0.04
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 2
Measured pKd: 3.51
Experiment series 3
Protein A protein: ARHGEF7
Protein A fragment: 794-803
Protein A site: PBM
Protein A sequence: NDPAWDETNL
Protein A construct: biotin-ttds-NDPAWDETNL
Protein B protein: Inadl
Protein B fragment: 1673-1763
Protein B site: PDZ_10
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.02
Holdup BI standard deviation: 0.04
Normalized holdup BI: 0.02
Normalized holdup BI standard deviation: 0.04
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 2
The affinity was below the detection threshold of the assay.
Experiment series 4
Protein A protein: ARHGEF7
Protein A fragment: 794-803
Protein A site: PBM
Protein A sequence: NDPAWDETNL
Protein A construct: biotin-ttds-NDPAWDETNL
Protein B protein: Inadl
Protein B fragment: 238-335
Protein B site: PDZ_2
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.02
Holdup BI standard deviation: 0.02
Normalized holdup BI: 0.02
Normalized holdup BI standard deviation: 0.02
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 2
The affinity was below the detection threshold of the assay.
Experiment series 5
Protein A protein: ARHGEF7
Protein A fragment: 794-803
Protein A site: PBM
Protein A sequence: NDPAWDETNL
Protein A construct: biotin-ttds-NDPAWDETNL
Protein B protein: Inadl
Protein B fragment: 359-469
Protein B site: PDZ_3
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.03
Holdup BI standard deviation: 0.03
Normalized holdup BI: 0.03
Normalized holdup BI standard deviation: 0.03
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 2
The affinity was below the detection threshold of the assay.
Experiment series 6
Protein A protein: ARHGEF7
Protein A fragment: 794-803
Protein A site: PBM
Protein A sequence: NDPAWDETNL
Protein A construct: biotin-ttds-NDPAWDETNL
Protein B protein: Inadl
Protein B fragment: 547-651
Protein B site: PDZ_4
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: -0.02
Holdup BI standard deviation: 0.05
Normalized holdup BI: -0.02
Normalized holdup BI standard deviation: 0.05
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 2
The affinity was below the detection threshold of the assay.
Experiment series 7
Protein A protein: ARHGEF7
Protein A fragment: 794-803
Protein A site: PBM
Protein A sequence: NDPAWDETNL
Protein A construct: biotin-ttds-NDPAWDETNL
Protein B protein: Inadl
Protein B fragment: 673-780
Protein B site: PDZ_5
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.02
Holdup BI standard deviation: 0.02
Normalized holdup BI: 0.02
Normalized holdup BI standard deviation: 0.02
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 2
The affinity was below the detection threshold of the assay.
Experiment series 8
Protein A protein: ARHGEF7
Protein A fragment: 794-803
Protein A site: PBM
Protein A sequence: NDPAWDETNL
Protein A construct: biotin-ttds-NDPAWDETNL
Protein B protein: Inadl
Protein B fragment: 1425-1530
Protein B site: PDZ_8
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: -0
Holdup BI standard deviation: 0.02
Normalized holdup BI: -0
Normalized holdup BI standard deviation: 0.03
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 2
The affinity was below the detection threshold of the assay.
Experiment series 9
Protein A protein: ARHGEF7
Protein A fragment: 794-803
Protein A site: PBM
Protein A sequence: NDPAWDETNL
Protein A construct: biotin-ttds-NDPAWDETNL
Protein B protein: Inadl
Protein B fragment: 1530-1615
Protein B site: PDZ_9
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: -0
Holdup BI standard deviation: 0.02
Normalized holdup BI: -0
Normalized holdup BI standard deviation: 0.02
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 2
The affinity was below the detection threshold of the assay.