Details of experiments between Q14155 and Q8IYB3
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: ARHGEF7
Protein A fragment: 178-251
Protein A site: SH3
Protein A construct: avi(biotin)-his6-MBP-TEVsite-SH3
Protein B protein: SRRM1
Protein B fragment: 1-904
Protein B construct: endogenous protein (Jurkat)
Average holdup BI: 0.29
Immobilized partner concentration in holdup experiment (10-6M): 10
P value (usually -log10(P Two-sided unpaired T-test), but double check the experimental details): 5.35
Experiment method: nHU_Jurkat
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022.06.29.
Experimental details: -log10(P Two-sided unpaired T-test (6 vs 3))
Number of measurements: 3
Measured pKd: 4.62
Experiment series 2
Protein A protein: ARHGEF7
Protein A fragment: 178-251
Protein A site: SH3
Protein A construct: avi(biotin)-his6-MBP-TEVsite-SH3
Protein B protein: SRRM1
Protein B fragment: 327-341
Protein B site: PxxP_1
Protein B sequence: TPPRRMPPPPRHRRS
Protein B construct: Biotinylated 15-mer peptide (crude)
Average holdup BI: 0.32
Holdup BI standard deviation: 0.01
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022.06.29.
Number of measurements: 2
Measured pKd: 4.45
Experiment series 3
Protein A protein: ARHGEF7
Protein A fragment: 178-251
Protein A site: SH3
Protein A construct: avi(biotin)-his6-MBP-TEVsite-SH3
Protein B protein: SRRM1
Protein B fragment: 365-379
Protein B site: PxxP_2
Protein B sequence: SRSRSPPKKPPKRTS
Protein B construct: Biotinylated 15-mer peptide (crude)
Average holdup BI: 0.07
Holdup BI standard deviation: 0.04
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022.06.29.
Number of measurements: 4
Measured pKd: 3.66
Experiment series 4
Protein A protein: ARHGEF7
Protein A fragment: 178-251
Protein A site: SH3
Protein A construct: avi(biotin)-his6-MBP-TEVsite-SH3
Protein B protein: SRRM1
Protein B fragment: 395-409
Protein B site: PxxP_3
Protein B sequence: PRRRHRPSPPATPPP
Protein B construct: Biotinylated 15-mer peptide (crude)
Average holdup BI: 0.12
Holdup BI standard deviation: 0.01
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022.06.29.
Number of measurements: 2
Measured pKd: 3.9
Experiment series 5
Protein A protein: ARHGEF7
Protein A fragment: 178-251
Protein A site: SH3
Protein A construct: avi(biotin)-his6-MBP-TEVsite-SH3
Protein B protein: SRRM1
Protein B fragment: 544-558
Protein B site: PxxP_4
Protein B sequence: GRRRRSPSPPPTRRR
Protein B construct: Biotinylated 15-mer peptide (crude)
Average holdup BI: 0.47
Holdup BI standard deviation: 0.03
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022.06.29.
Number of measurements: 2
Measured pKd: 4.74
Experiment series 6
Protein A protein: ARHGEF7
Protein A fragment: 178-251
Protein A site: SH3
Protein A construct: avi(biotin)-his6-MBP-TEVsite-SH3
Protein B protein: SRRM1
Protein B fragment: 557-571
Protein B site: PxxP_5
Protein B sequence: RRRSPSPAPPPRRRR
Protein B construct: Biotinylated 15-mer peptide (crude)
Average holdup BI: 0.54
Holdup BI standard deviation: 0.03
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022.06.29.
Number of measurements: 2
Measured pKd: 4.87
Experiment series 7
Protein A protein: ARHGEF7
Protein A fragment: 178-251
Protein A site: SH3
Protein A construct: avi(biotin)-his6-MBP-TEVsite-SH3
Protein B protein: SRRM1
Protein B fragment: 567-581
Protein B site: PxxP_6
Protein B sequence: PRRRRTPTPPPRRRT
Protein B construct: Biotinylated 15-mer peptide (crude)
Average holdup BI: 0.47
Holdup BI standard deviation: 0.02
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022.06.29.
Number of measurements: 4
Measured pKd: 4.75
Experiment series 8
Protein A protein: ARHGEF7
Protein A fragment: 178-251
Protein A site: SH3
Protein A construct: avi(biotin)-his6-MBP-TEVsite-SH3
Protein B protein: SRRM1
Protein B fragment: 576-590
Protein B site: PxxP_7
Protein B sequence: PPRRRTPSPPPRRRS
Protein B construct: Biotinylated 15-mer peptide (crude)
Average holdup BI: 0.41
Holdup BI standard deviation: 0.04
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022.06.29.
Number of measurements: 4
Measured pKd: 4.63
Experiment series 9
Protein A protein: ARHGEF7
Protein A fragment: 178-251
Protein A site: SH3
Protein A construct: avi(biotin)-his6-MBP-TEVsite-SH3
Protein B protein: SRRM1
Protein B fragment: 600-614
Protein B site: PxxP_8
Protein B sequence: IQRRYSPSPPPKRRT
Protein B construct: Biotinylated 15-mer peptide (crude)
Average holdup BI: 0.19
Holdup BI standard deviation: 0.05
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022.06.29.
Number of measurements: 4
Measured pKd: 4.13
Experiment series 10
Protein A protein: ARHGEF7
Protein A fragment: 178-251
Protein A site: SH3
Protein A construct: avi(biotin)-his6-MBP-TEVsite-SH3
Protein B protein: SRRM1
Protein B fragment: 611-625
Protein B site: PxxP_9
Protein B sequence: KRRTASPPPPPKRRA
Protein B construct: Biotinylated 15-mer peptide (crude)
Average holdup BI: 0.2
Holdup BI standard deviation: 0.01
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022.06.29.
Number of measurements: 2
Measured pKd: 4.17
Experiment series 11
Protein A protein: ARHGEF7
Protein A fragment: 178-251
Protein A site: SH3
Protein A construct: avi(biotin)-his6-MBP-TEVsite-SH3
Protein B protein: SRRM1
Protein B fragment: 612-626
Protein B site: PxxP_10
Protein B sequence: RRTASPPPPPKRRAS
Protein B construct: Biotinylated 15-mer peptide (crude)
Average holdup BI: 0.34
Holdup BI standard deviation: 0.05
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022.06.29.
Number of measurements: 2
Measured pKd: 4.5
Experiment series 12
Protein A protein: ARHGEF7
Protein A fragment: 178-251
Protein A site: SH3
Protein A construct: avi(biotin)-his6-MBP-TEVsite-SH3
Protein B protein: SRRM1
Protein B fragment: 621-635
Protein B site: PxxP_11
Protein B sequence: PKRRASPSPPPKRRV
Protein B construct: Biotinylated 15-mer peptide (crude)
Average holdup BI: 0.18
Holdup BI standard deviation: 0.04
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022.06.29.
Number of measurements: 4
Measured pKd: 4.09
Experiment series 13
Protein A protein: ARHGEF7
Protein A fragment: 178-251
Protein A site: SH3
Protein A construct: avi(biotin)-his6-MBP-TEVsite-SH3
Protein B protein: SRRM1
Protein B fragment: 759-773
Protein B site: PxxP_12
Protein B sequence: PAAKKPPAPPSPVQS
Protein B construct: Biotinylated 15-mer peptide (crude)
Average holdup BI: 0.02
Holdup BI standard deviation: 0.06
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022.06.29.
Number of measurements: 2
The affinity was below the detection threshold of the assay.
Experiment series 14
Protein A protein: ARHGEF7
Protein A fragment: 178-251
Protein A site: SH3
Protein A construct: avi(biotin)-his6-MBP-TEVsite-SH3
Protein B protein: SRRM1
Protein B fragment: 776-790
Protein B site: PxxP_13
Protein B sequence: PSTNWSPAVPVKKAK
Protein B construct: Biotinylated 15-mer peptide (crude)
Average holdup BI: 0.02
Holdup BI standard deviation: 0.01
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022.06.29.
Number of measurements: 2
The affinity was below the detection threshold of the assay.
Experiment series 15
Protein A protein: ARHGEF7
Protein A fragment: 178-251
Protein A site: SH3
Protein A construct: his6-MBP-TEVsite-SH3
Protein B protein: SRRM1
Protein B fragment: 576-590
Protein B site: PxxP_7
Protein B sequence: PPRRRTPSPPPRRRS
Protein B construct: Bio E E Ado Ado-PPRRRTPSPPPRRRS-CONH2
Average holdup BI: 0.02
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/17
Number of measurements: 1
The affinity was below the detection threshold of the assay.