Details of experiments between Q14005 and P0DTC9
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: IL16
Protein A fragment: 210-306
Protein A site: PDZ_1
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: SARS-Cov2-N
Protein B fragment: 410-419
Protein B site: PBM
Protein B sequence: SMSSADSTQA
Protein B construct: Biotin-(PEG)3-QQSMSSADSTQA
Average holdup BI: -0.05
Holdup BI standard deviation: 0.04
Immobilized partner concentration in holdup experiment (10-6M): 20
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 2
The affinity was below the detection threshold of the assay.
Experiment series 2
Protein A protein: IL16
Protein A fragment: 344-452
Protein A site: PDZ_2
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: SARS-Cov2-N
Protein B fragment: 410-419
Protein B site: PBM
Protein B sequence: SMSSADSTQA
Protein B construct: Biotin-(PEG)3-QQSMSSADSTQA
Average holdup BI: -0.08
Holdup BI standard deviation: 0.06
Immobilized partner concentration in holdup experiment (10-6M): 20
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 2
The affinity was below the detection threshold of the assay.
Experiment series 3
Protein A protein: IL16
Protein A fragment: 1106-1201
Protein A site: PDZ_3
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: SARS-Cov2-N
Protein B fragment: 410-419
Protein B site: PBM
Protein B sequence: SMSSADSTQA
Protein B construct: Biotin-(PEG)3-QQSMSSADSTQA
Average holdup BI: -0.01
Holdup BI standard deviation: 0
Immobilized partner concentration in holdup experiment (10-6M): 20
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 2
The affinity was below the detection threshold of the assay.
Experiment series 4
Protein A protein: IL16
Protein A fragment: 1203-1332
Protein A site: PDZ_4
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: SARS-Cov2-N
Protein B fragment: 410-419
Protein B site: PBM
Protein B sequence: SMSSADSTQA
Protein B construct: Biotin-(PEG)3-QQSMSSADSTQA
Average holdup BI: -0.01
Holdup BI standard deviation: 0.01
Immobilized partner concentration in holdup experiment (10-6M): 20
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 2
The affinity was below the detection threshold of the assay.