Details of experiments between Q14005 and O75912
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: IL16
Protein A fragment: 210-306
Protein A site: PDZ_1
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: DGKI
Protein B fragment: 1056-1065
Protein B site: PBM
Protein B sequence: IGHEDLETAV
Protein B construct: biotin-ado-ado-IGHEDLETAV
Average holdup BI: 0.24
Normalized holdup BI: 0.24
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Experimental details: Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality causing a systematic error in reported affinities.
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 4.26
Experiment series 2
Protein A protein: IL16
Protein A fragment: 1203-1332
Protein A site: PDZ_4
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: DGKI
Protein B fragment: 1056-1065
Protein B site: PBM
Protein B sequence: IGHEDLETAV
Protein B construct: biotin-ado-ado-IGHEDLETAV
Average holdup BI: 0.06
Normalized holdup BI: 0.06
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Experimental details: Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality causing a systematic error in reported affinities.
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 3.52
Experiment series 3
Protein A protein: IL16
Protein A fragment: 1106-1201
Protein A site: PDZ_3
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: DGKI
Protein B fragment: 1056-1065
Protein B site: PBM
Protein B sequence: IGHEDLETAV
Protein B construct: biotin-ado-ado-IGHEDLETAV
Average holdup BI: -0.1
Normalized holdup BI: -0.1
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Experimental details: Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality causing a systematic error in reported affinities.
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.