Details of experiments between Q14005 and A6NIM6
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: IL16
Protein A fragment: 210-306
Protein A site: PDZ_1
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: SLC15A5
Protein B fragment: 570-579
Protein B site: PBM
Protein B sequence: SSIDLWETAL
Protein B construct: biotin-ttds-SSIDLWETAL
Average holdup BI: 0.02
Immobilized partner concentration in holdup experiment (10-6M): 3.5
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 2
Protein A protein: IL16
Protein A fragment: 344-452
Protein A site: PDZ_2
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: SLC15A5
Protein B fragment: 570-579
Protein B site: PBM
Protein B sequence: SSIDLWETAL
Protein B construct: biotin-ttds-SSIDLWETAL
Average holdup BI: -0.14
Immobilized partner concentration in holdup experiment (10-6M): 3.5
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 3
Protein A protein: IL16
Protein A fragment: 1106-1201
Protein A site: PDZ_3
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: SLC15A5
Protein B fragment: 570-579
Protein B site: PBM
Protein B sequence: SSIDLWETAL
Protein B construct: biotin-ttds-SSIDLWETAL
Average holdup BI: -0.11
Immobilized partner concentration in holdup experiment (10-6M): 3.5
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 4
Protein A protein: IL16
Protein A fragment: 1203-1332
Protein A site: PDZ_4
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: SLC15A5
Protein B fragment: 570-579
Protein B site: PBM
Protein B sequence: SSIDLWETAL
Protein B construct: biotin-ttds-SSIDLWETAL
Average holdup BI: -0.1
Immobilized partner concentration in holdup experiment (10-6M): 3.5
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 5
Protein A protein: IL16
Protein A fragment: 210-306
Protein A site: PDZ_1
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: SLC15A5
Protein B fragment: 570-579
Protein B site: PBM
Protein B sequence: SSIDLWETAL
Protein B construct: biotin-ttds-SSIDLWETAL
Average holdup BI: 0.17
Holdup BI standard deviation: 0.04
Normalized holdup BI: 0.17
Normalized holdup BI standard deviation: 0.04
Immobilized partner concentration in holdup experiment (10-6M): 11
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 2
Measured pKd: 4.29
Experiment series 6
Protein A protein: IL16
Protein A fragment: 1106-1201
Protein A site: PDZ_3
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: SLC15A5
Protein B fragment: 570-579
Protein B site: PBM
Protein B sequence: SSIDLWETAL
Protein B construct: biotin-ttds-SSIDLWETAL
Average holdup BI: 0
Holdup BI standard deviation: 0.01
Normalized holdup BI: 0
Normalized holdup BI standard deviation: 0.01
Immobilized partner concentration in holdup experiment (10-6M): 11
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 2
The affinity was below the detection threshold of the assay.
Experiment series 7
Protein A protein: IL16
Protein A fragment: 1203-1332
Protein A site: PDZ_4
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: SLC15A5
Protein B fragment: 570-579
Protein B site: PBM
Protein B sequence: SSIDLWETAL
Protein B construct: biotin-ttds-SSIDLWETAL
Average holdup BI: 0.03
Holdup BI standard deviation: 0.01
Normalized holdup BI: 0.03
Normalized holdup BI standard deviation: 0.01
Immobilized partner concentration in holdup experiment (10-6M): 11
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 2
The affinity was below the detection threshold of the assay.
Experiment series 8
Protein A protein: IL16
Protein A fragment: 210-306
Protein A site: PDZ_1
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: SLC15A5
Protein B fragment: 570-579
Protein B site: PBM
Protein B sequence: SSIDLWETAL
Protein B construct: biotin-ttds-SSIDLWETAL
Average holdup BI: 0.13
Normalized holdup BI: 0.13
Immobilized partner concentration in holdup experiment (10-6M): 11
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Experimental details: not reported internal standards of holdup layout #5
Number of measurements: 1
Measured pKd: 4.16
Experiment series 9
Protein A protein: IL16
Protein A fragment: 1106-1201
Protein A site: PDZ_3
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: SLC15A5
Protein B fragment: 570-579
Protein B site: PBM
Protein B sequence: SSIDLWETAL
Protein B construct: biotin-ttds-SSIDLWETAL
Average holdup BI: -0.05
Normalized holdup BI: -0.05
Immobilized partner concentration in holdup experiment (10-6M): 11
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Experimental details: not reported internal standards of holdup layout #5
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 10
Protein A protein: IL16
Protein A fragment: 1203-1332
Protein A site: PDZ_4
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: SLC15A5
Protein B fragment: 570-579
Protein B site: PBM
Protein B sequence: SSIDLWETAL
Protein B construct: biotin-ttds-SSIDLWETAL
Average holdup BI: -0.04
Normalized holdup BI: -0.04
Immobilized partner concentration in holdup experiment (10-6M): 11
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Experimental details: not reported internal standards of holdup layout #5
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 11
Protein A protein: IL16
Protein A fragment: 1203-1332
Protein A site: PDZ_4
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: SLC15A5
Protein B fragment: 570-579
Protein B site: PBM
Protein B sequence: SSIDLWETAL
Protein B construct: Biotin-ado-ado-SSIDLWETAL
Average holdup BI: -0.01
Normalized holdup BI: -0.01
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Experimental details: Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality. Not reported internal standards of holdup layout #6
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 12
Protein A protein: IL16
Protein A fragment: 1106-1201
Protein A site: PDZ_3
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: SLC15A5
Protein B fragment: 570-579
Protein B site: PBM
Protein B sequence: SSIDLWETAL
Protein B construct: Biotin-ado-ado-SSIDLWETAL
Average holdup BI: 0.04
Normalized holdup BI: 0.04
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Experimental details: Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality. Not reported internal standards of holdup layout #6
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 13
Protein A protein: IL16
Protein A fragment: 210-306
Protein A site: PDZ_1
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: SLC15A5
Protein B fragment: 570-579
Protein B site: PBM
Protein B sequence: SSIDLWETAL
Protein B construct: Biotin-ado-ado-SSIDLWETAL
Average holdup BI: 0.17
Normalized holdup BI: 0.17
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Experimental details: Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality. Not reported internal standards of holdup layout #6
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 4.08