Details of experiments between the fragments Q13884~107-196 and P27816~1143-1152
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: SNTB1
Protein A fragment: 107-196
Protein A site: PDZ
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: MAP4
Protein B fragment: 1143-1152
Protein B site: PBM
Protein B sequence: LDSQIQETSI
Protein B construct: biotin-ttds-LDSQIQETSI
Average holdup BI: 0.48
Holdup BI standard deviation: 0.08
Normalized holdup BI: 0.57
Normalized holdup BI standard deviation: 0.1
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 2
Measured pKd: 4.93