Details of experiments between the fragments Q13884~107-196 and P13569~1471-1480
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: SNTB1
Protein A fragment: 107-196
Protein A site: PDZ
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: CFTR
Protein B fragment: 1471-1480
Protein B site: PBM
Protein B sequence: TEEEVQDTRL
Protein B construct: biotin-ttds-TEEEVQDTRL
Average holdup BI: 0.18
Holdup BI standard deviation: 0.07
Normalized holdup BI: 0.21
Normalized holdup BI standard deviation: 0.09
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 2
Measured pKd: 4.2
Experiment series 2
Protein A protein: SNTB1
Protein A fragment: 107-196
Protein A site: PDZ
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: CFTR
Protein B fragment: 1471-1480
Protein B site: PBM
Protein B sequence: TEEEVQDTRL
Protein B construct: Biotin-ado-ado-TEEEVQDTRL
Average holdup BI: 0.26
Normalized holdup BI: 0.29
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Experimental details: Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality. Not reported internal standards of holdup layout #6
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 4.39