Details of experiments between the fragments Q13574~919-928 and Q9P202~136-226
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: DGKZ
Protein A fragment: 919-928
Protein A site: PBM
Protein A sequence: IQREDQETAV
Protein A construct: biotin-ttds-IQREDQETAV
Protein B protein: DFNB31
Protein B fragment: 136-226
Protein B site: PDZ_1
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.35
Immobilized partner concentration in holdup experiment (10-6M): 6
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 5.06
Experiment series 2
Protein A protein: DGKZ
Protein A fragment: 919-928
Protein A site: PBM
Protein A sequence: IQREDQETAV
Protein A construct: biotin-ttds-IQREDQETAV
Protein B protein: DFNB31
Protein B fragment: 136-226
Protein B site: PDZ_1
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.43
Holdup BI standard deviation: 0.08
Normalized holdup BI: 0.43
Normalized holdup BI standard deviation: 0.08
Immobilized partner concentration in holdup experiment (10-6M): 13.5
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 2
Measured pKd: 4.81
Experiment series 3
Protein A protein: DGKZ
Protein A fragment: 919-928
Protein A site: PBM
Protein A sequence: IQREDQETAV
Protein A construct: biotin-ttds-IQREDQETAV
Protein B protein: DFNB31
Protein B fragment: 136-226
Protein B site: PDZ_1
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.53
Normalized holdup BI: 0.53
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Experimental details: not reported internal standards of holdup layout #6
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 4.84