Details of experiments between the fragments Q13574~919-928 and Q14160~1-1630
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: DGKZ
Protein A fragment: 919-928
Protein A site: PBM
Protein A sequence: IQREDQETAV
Protein A construct: biotin-ttds-IQREDQETAV
Protein B protein: SCRIB
Protein B fragment: 1-1630
Protein B construct: endogenous protein (Jurkat)
Experiment method: nHU-WB_Jurkat
Details of affinity fitting: Hill equation (8 point titration) h=0.6
Date of measurement: 2022.08.03.
PUBMED ID of publication: 36542723
Number of measurements: 3
Measured pKd: 6.4
Experiment series 2
Protein A protein: DGKZ
Protein A fragment: 919-928
Protein A site: PBM
Protein A sequence: IQREDQETAV
Protein A construct: biotin-ttds-IQREDQETAV
Protein B protein: SCRIB
Protein B fragment: 1-1630
Protein B construct: endogenous protein (Jurkat)
Experiment method: nHU-WB_Jurkat
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022.08.03.
PUBMED ID of publication: 36542723
Number of measurements: 3
Measured pKd: 6.42